README for Figure S8C.csv 
*** This file contains the raw data obtained on growth of NT135 Escherichia coli cells transformed with empty pUC19 plasmid 
or with an insert containing the hns promoter followed by the hns, rok, srok or rok ?75-96 coding sequence 
represented in Figure S8C of 
Article: The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues
Authors: Erkelens, Qin, van Erp, Miguel-Arribas, Abia, Keek, Markus, Cajili, Schwab, Meijer and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl; wmeijer@cbm.csic.es

Legend Figure S8: Rok and sRok cannot complement a Δhns phenotype, but Rok Δ75-96 can A) Complementation of bgl 
operon repression tested on MacConkey agar plates supplemented with 0.4% salicin. E. coli NT135 cells were 
transformed with either an empty pUC19 plasmid or with an insert containing the hns promoter followed by the hns, 
rok, srok or rokΔ75-96 coding sequence. B) Complementation of bgl operon repression tested on BTB indicator plates 
supplemented with 0.5% salicin, using the same cells as in A. C) Growth curves in LB medium over time. Data are 
plotted as mean values and the error bars represent the standard deviation of three independent growth curves. 
Dashed lines are lines to guide the eye. 

*** The data were obtained using growth measurements (OD600) as described in the associated article. 

***The data labeled overview represents the values plotted in the graph of figure S8C. The raw data contains the individual replicates.
Column A: Time in minutes after start of culture
Column B: Optical density of culture with NT135 transformed with empty plasmid
Column C: Optical density of culture with NT135 transformed with H-NS plasmid
Column D: Optical density of culture with NT135 transformed with Rok plasmid
Column E: Optical density of culture with NT135 transformed with sRok plasmid
Column F: Optical density of culture with NT135 transformed with Rok ?75-96 plasmid
Followed by the replicates of parental MG1655 Escherichia coli cells in columns B, C and D. 








